These proprietary methods can facilitate over-sized DNA cloning for surface-display and/or produce soluble exogenous protein of 10 -150 kDa. With both methods, the exogenous protein is associated with phage replication life cycle the same as intact domain refolding in E.coli host plasma and retaining desired original biological activity. These characteristics make T4 and T4-like phage a cutting-edge, attractive protocol for consistent and large-scale production of medical biological peptides and proteins. As a result, we have developed a complete portfolio of T4 phage display and soluble protein expression Nano-platform tools based on these inventions.
T4-S-GPDS (Surface Gene-Protein Display System) on phage capsid surface display
Using T4-S-GPDS method, foreign proteins or peptides can be displayed on phage particle surface in high copy number as intact domain with desired biological activity
T4-M-SPES (in Medium Soluble Protein Expression System) for soluble protein production
T4-M-SPES method produces soluble and refold form of the foreign protein