These proprietary methods can facilitate over-sized DNA cloning for surface-display and/or produce soluble exogenous protein of 10 -150 kDa. With both methods, the exogenous protein is associated with phage replication life cycle the same as intact domain refolding in E.coli host plasma and retaining desired original biological activity. These characteristics make T4 and T4-like phage a cutting-edge, attractive protocol for consistent and large-scale production of medical biological peptides and proteins.  As a result, we have developed a complete portfolio of T4 phage display and soluble protein expression Nano-platform tools based on these inventions.